LCMS - Vitamin B1 & B6

Vitamin B1 & B6 reagents set

SKU: 1020 KIT M VB1B6

The Vitamin B1 & B6 reagents set from diagnotix • •• is based on Waters’ published TCA method – market leader in mass spectrometry. The method contains a lower TCA concentration which benefits your system.

Components

Vitamin B1 (Thiamine diphosphate)
Vitamin B6 (Pyridoxal-5’-phosphate)

At a glance

Matrix: Whole blood
Technique: LC-MS/MS
Internal Standard: Thiamine diphosphate-D3, Pyridoxal-5’-phosphate-D3

Included in kit (300 assays):

1022a CAL M VB1B6, Calibrator Set (Calibrator 1 – 6), 6 x 2 x 250 µl
1029 M VB1B6, Internal Standard D3, 3 x 6 ml
1030 M VB1B6, Deproteinization Solution, 3 x 46 ml
1031 M VB1B6, Mobile Phase I, 1 x 500 ml
1032 M VB1B6, Mobile Phase II, 1 x 250 ml
10201 M VB1B6, Autosampler Washing Solution, 1 x 1000 ml

Water-soluble B Vitamins are important cofactors in cell metabolism. Two water-soluble vitamins with clinical relevance are Vitamins B1 and B6. Thiamine Diphosphate (TDP) is the biologically active form of Vitamin B1 and is required for various metabolic functions. Prolonged deficiency can cause Beriberi, a debilitating neurological disease. Pyridoxal 5- phosphate (PLP) is the biologically active form of Vitamin B6 and is a coenzyme for a number of transamination reactions. It plays critical roles in chronic disease and pro-inflammatory response. Additionally, both Vitamin B6 and B1 have also been linked to increased survival rates in the elderly.

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Chromatograms

Technical data

Applicable for at Least:	Waters TQ-Sµ, Shimadzu LC-8050, Sciex Citrine
Limit of Quantification; Vitamin B1 / Vitamin B6 (µmol/l)	6.43 / 8.00
Linearity; Vitamin B1 / Vitamin B6 (µmol/l)	8000 / 3000
Accuracy	Passes (<15% CV)
Simple Precision; Vitamin B1 / Vitamin B6 (CV %)	2.4 / 3.4
Complex Precision; Vitamin B1 / Vitamin B6 (CV %)	4.7 / 4.9
Ionization	Electrospray
MS/MS mode	Positive
Sample Preparation	50 µl Whole blood (EDTA and Heparin) → 50 µl IS → 400 µl deprot → shaker → centrifuge → inject
Preferred Column	Xbridge BEH C18 XP Column 2.5 µm, 3mm x 75 mm
Analysis Time	2.5 minutes
Flow Rate	0.6 ml/min
Injection Volume	10-20 µl
Validation Report Available	Yes

Please Note:

Information as presented freely on this website is not sufficient for proper operation of these medical devices. Please refer to the applicable instructions for use and all accompanying information such as warning notices. All necessary documentation can be found in the download centre section of this website.

For other LC-MS/MS systems than listed above, please contact us to discuss the possibilities.