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LCMS - Psychotropic Medication 1, Tricyclic Antidepressants TDM
Psychotropic Medication 1 (TCA) Reagent Kit
The advantages of the Psychotropic Medication 1 Reagent kit for LC-MS/MS:
- Balanced panel for cost reducing and optimal chromatography
- Simple and improved column life sample preparation
- No SPE or LLE needed
- All important compounds has their own internal standard (Except E-OH- and Z-OH-Nortriptyline)
- 25 µl sample required (compared to 500 µl for SPE-HPLC-UV). Less might be even possible.
Components
- Amitriptyline
- Nortriptyline
- Z-10-OH-nortryptiline
- E-10-OH-nortyptiline
- Clozapine
- Norclozapine
- Clomipramine
- Norclomipramine
- Imipramine
- Desipramine
- Zuclopentixol
At a glance
- Matrix: Serum
- Technique: LC-MS/MS
- Internal Standard: Amitriptyline-D3, Clozapine D4, Clomipramine D3, Desipramine D4, Imipramine D4, Nortriptyline D3 (also used for E-10-OH-Nortriptyline & Z-10-OH-Nortriptyline) & Zuclopenthixol-D4.
Intended Use
The Diagnotix Psychotropic Medication 1 LC-MS Reagent Kit is designed to determine the concentration of one or more of the listed analytes and metabolites in serum or plasma as a basis for further diagnostics.
Technical Benefits
- Balanced panel: The most abundant compounds and their common metabolites are chosen to ensure that the panel is not too large, but still meets the challenges of routine use. The larger the panel, the more costs are involved to include all analytes and their isotopes, but also more trade-offs must be made for chromatographic optimization.
- Unique panel: At the request of Dutch specialists, we have added E- and Z-OH nortriptyline to the panel.
- Column and LC-MS friendly: A 40-fold deproteinization solution is used, which ensures extreme sample cleaning. Also, only a small injection volume is required for most systems.
- Cost-effective and fast automatable sample preparation: No more SPE or LLE is needed. SPE for HPLC-UV analyses are used to extend the life of the column and reduce background noise from possible interfering drugs of abuse. As mentioned in 3. the sample is diluted 40 times and essentially all protein is removed. Furthermore, the use of LC-MS ensures that no co-eluting molecules interfere with your compound of interest, because they do not share the same mass transitions.
Why moving over from HPLC to LC-MS?
By switching to LC-MS, you immediately save on the use of SPE, longer sample preparation and therefore labor costs. In addition, the runtime of your method is extended and you do not have to worry about interfering other drugs, as long as they do not share the same mass transitions.
And because baseline separations are no longer necessary and the mobile phases do not have to contain high concentration buffers, you benefit from a much longer column life.
Especially interesting for laboratories that:
I. Have regular (large) batches of TCA patient samples and have a reliable automatable workflow.
II. Already have an LC-MS system, but still perform TDM or TCA analyses on HPLC-UV machines. Eliminating the use of your HPLC-UV system will immediately improve operational efficiency and reduce overall maintenance costs.
Contact Us for More Information
Interested in learning more about our Psychotropic Medication 1 solution for Tryciclic Antidepressants? Contact us via the contact form below. We looking forward to hear from you!
Included in kit (300 assays)
- 4051 CAL P PS1, Calibrator Set (Calibrator 1 – 6), 6 x 2 x 500 µl
- 4059 P PS1, Deproteinization Solution + Internal Standard, 3 x 100 ml
- 4064 P PS1, Mobile Phase I, 1 x 500 ml
- 4065 P PS1, Mobile Phase II, 1 x 500 ml
- 4066 P PS1, Autosampler Washing Solution, 1 x 1000 ml
Clinical Relevance
Tricyclic antidepressants (amitriptyline, nortriptyline, clomipramine, imipramine and desipramine) and antipsychotics (clozapine). Therapeutic Drug Monitoring (TDM) is based on the assumption that there is a relationship between the blood concentration and clinical effect (therapeutic improvement and adverse effects). It also assumes there is a concentration range of the drug which is characterized by maximal effectiveness and maximal safety, the “therapeutic window”. The Diagnotix kit for measuring tricyclic antidepressants (TCAs) and antipsychotics (PS1) includes the TCAs amitriptyline, nortriptyline, clomipramine, imipramine and desipramine (including the drugs’ metabolites) and the antipsychotic clozapine (including the metabolite norclozapine).
The TCAs amitriptyline, nortriptyline, clomipramine, imipramine and desipramine are used for the treatment of several types of depression, obsessive compulsive disorder, neuropathic pain, nocturnal enuresis, and for the prophylactic treatment of chronic tension type headache and migraine. TCAs have a pronounced inter-individual pharmacokinetic variability and a narrow therapeutic window. Studies on the relation between blood concentration and clinical improvement have supported the relation for the TCAs. Systematic reviews and meta-analyses led to convincing evidence of a significant relationship between clinical outcomes and plasma concentrations for the TCAs which are associated with a high probability of response.
Chromatograms
Technical data
| Applicable for at Least: | Waters TQ-Sµ, Shimadzu LC-8050, Sciex Citrine |
|---|---|
| Limit of Quantification; Amitriptyline / Clomipramine / Clozapine / Desipramine / Imipramine (µg/l) | 3.66 / 6.12 / 11.05 / 4.27 / 4.97 |
| Norclomipramine / Norclozapine / Nortriptyline / E-10-OH-Nortriptyline / Z-10-OH-Nortriptyline / Zuclopenthixol (µg/l) | 4.31 / 6.46 / 5.65 / 15.49 / 4.10 / 2.63 |
| Linearity; Amitriptyline / Clomipramine / Clozapine / Desipramine / Imipramine (µg/l) | 1800 / 1400 / 2000 / 1230 / 2000 |
| Norclomipramine / Norclozapine / Nortriptyline / E-10-OH-Nortriptyline / Z-10-OH-Nortriptyline / Zuclopenthixol (µg/l) | 1175 / 2650 / 1250 / 1050 / 288 / 290 |
| Accuracy | Passes (<15% CV) |
| Simple Precision; Amitriptyline / Clomipramine / Clozapine / Desipramine / Imipramine (µg/l) | < 3.7 / < 2.0 / < 2.4 / < 2.8 / < 4.1 |
| Norclomipramine / Norclozapine / Nortriptyline / E-10-OH-Nortriptyline / Z-10-OH-Nortriptyline / Zuclopenthixol (µg/l) | < 2.3 / < 3.3 / < 6.6 / < 4.0 / < 5.3 / < 10.9 |
| Complex Precision; Amitriptyline / Clomipramine / Clozapine / Desipramine / Imipramine (CV %) | < 8.0 / < 6.4 / < 4.9 / < 5.5 / < 7.1 |
| Norclomipramine / Norclozapine / Nortriptyline / E-10-OH-Nortriptyline / Z-10-OH-Nortriptyline / Zuclopenthixol (CV %) | < 6.8 / < 5.0 / < 10.6 / < 8.1 / < 8.1 / < 13.9 |
| Ionization | Electrospray |
| MS/MS mode | Positive |
| Sample Preparation | 25 µl sample → 1 ml deprot + IS → centrifuge → inject |
| Preferred Column | Acquity UPLC HSS T3 1,8 µm 2.1x100 mm |
| Analysis Time | 7 min |
| Flow Rate | 0.4 ml/min |
| Injection Volume | 2-20 µl |
| Validation Report Available | Yes |
Please Note:
Information as presented freely on this website is not sufficient for proper operation of these medical devices. Please refer to the applicable instructions for use and all accompanying information such as warning notices. All necessary documentation can be found in the download centre section of this website.
For other LC-MS/MS systems than listed above, please contact us to discuss the possibilities.
Product downloads
More info?
Contact us for more information about this product using the contact form or the contact information below.
Contact details
De Plassen 4
9902 SE Appingedam
The Netherlands
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