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Methylmalonic acid (MMA) LC-MS
Methylmalonic Acid (MMA) LC-MS Reagens Kit
Advantages of the Diagnotix Methylmalonic (MMA) LC-MS method:
- Reversed Phase method with excellent separation between MMA and Succinyl Acid
- No need for derivatisation and positive mode measurements
- MMA-D3 internal standard lyophilized
- Six-points calibrator set in human serum
- Complex Precision CV is < 5%
- Relative long column life time
Component:
- Methylmalonic Acid (Methylmalonate, MMA)
At a glance:
- Matrix: serum/plasma
- Technique: LC-MS/MS
- Internal Standard: Methylmalonic Acid-D3
Intended use
The Diagnotix MMA LC-MS kit is developed to determine the patient's MMA concentration as a basis for further diagnostics.
Technical benefits
- Based on Reversed-Phase LC-MS/MS technology. LC-MS/MS allows the possibility to measure MMA from blood.
- Stable retention time while having complete separation of MMA and its isobar, succinic acid.
- The simple setup of the method allows for easy integration of other methodologies before or after, on the same LC-MS/MS system, without the need for extended conditioning time.
- Due to the chromatographic stability you achieve high data quality and win time in data-analysis.
- Refer to the technical details for the outstanding validation scores.
Why choosing for blood sampling over urine sampling
Serum samples are generally used for MMA determination, as this matrix is used for parallel cobalamin level tests. The advantage of determination from serum therefore is the sample availability. Furthermore nutrition seems to have less influence on the MMA serum level than in case with urine. For determination in urine additional measurement of creatinine is also necessary.
Especially interesting for: Laboratories running large numbers of samples once or more times per week
Contact Us for More Information
Interested in learning more about our MMA analysis? Contact us via the contact form below. We looking forward to hear from you!
Included in kit (300 assays):
- 1001 CAL M MMA, Calibrator Set (Calibrator 1 – 6), 6 x 2 x 500 µl
- 1008 M MMA, Internal Standard, 3 x 6 ml
- 1009 M MMA, Deproteinization Solution, 3 x 55 ml
- 1010a M MMA, Mobile Phase I, 1 x 500 ml
- 1011 M MMA, Mobile Phase II, 1 x 300 ml
- 1012 M MMA, Solvent, 3 x 30 ml
- 10001 M MMA, Autosampler Washing Solution, 1 x 1000 ml
Clinical relevance
Methylmalonic acid (MMA) is an organic acid of which the blood levels are usually raised in case of a B12 deficiency. Adenosylcobalamin – one of the two active forms of B12 – is a cofactor of the enzyme L-methylmalonyl-CoA- mutase, which converts L-methylmalonyl-Coa into succinyl-CoA. If adenosylcobalamin is lacking, excess D-methylmalonyl-CoA (precursor of Lmethylmalonyl-CoA) is converted into methylmalonic acid(MMA) which causes raised blood levels of MMA. In short: a B12 deficiency (usually) causes high MMA. High MMA serum values are also found in people with renal insufficiency, hypovolemia (decreased volume of circulating blood) and intestinal bacterial overgrowth. In these cases MMA levels cannot be used to diagnose B12 deficiency though a B12 deficiency might simultaneously exist. In the case of kidney disease (or hypovolemia) MMA levels in urine can be tested.
See also
- Methylmalonic Acid calibrator set (6 x 2 x 500 µl)
- Methylmalonic Acid Control set (3 x 3 x 500 µl)
- Methylmalonic Acid Internal Standard (10 x 6 ml)
- Methylmalonic acid solution (10 µg/ml) (1 x 1 ml)
- Methylmalonic acid-D3 solution (10 µg/ml) (1 x 1 ml)
- Large quantities of Calibrators or Controls (for HPLC or LC-MS)
- Custom made Calibrators & Controls
Chromatograms
Technical data
| Applicable for at Least: | Waters TQ-Sµ, Shimadzu LC-8050, Sciex Citrine |
|---|---|
| Limit of Quantification (nmol/l) | 33.4 |
| Linearity (nmol/l) | 5000 |
| Accuracy | Passes (<15% CV) |
| Simple Precision (CV %) | 2.0 |
| Complex Precision (CV %) | 4.9 |
| Ionization | Electrospray |
| MS/MS mode | Negative |
| Sample Preparation | 100 µl sample → 50 µl IS → 400 µl deprot → centrifuge → evaporate/reconstitute → inject |
| Preferred Column | Acquity UPLC HSS T3 100 A 1,8 µm 2,1 mm x 100 mm |
| Analysis Time | 6 minutes |
| Flow Rate | 0.2 ml/min |
| Injection Volume | 10-20 µl |
| Validation Report Available | Yes |
Please Note:
Information as presented freely on this website is not sufficient for proper operation of these medical devices. Please refer to the applicable instructions for use and all accompanying information such as warning notices. All necessary documentation can be found in the download centre section of this website.
For other LC-MS/MS systems than listed above, please contact us to discuss the possibilities.
Included in this set
Product downloads
More info?
Contact us for more information about this product using the contact form or the contact information below.
Contact details
De Plassen 4
9902 SE Appingedam
The Netherlands
Related products
Methylmalonic acid (MMA) Control Set
-
Control set of three different levels to guarantee the quality of the Methylmalonic acid (MMA, methylmalonate ) determinations in serum, plasma or urine.
Methylmalonic acid (MMA-D3) Internal Standard (box of 10 x 6 ml)
- Each box contains 10 vials of 6 mL, providing sufficient material for up to 120 mL of serum samples.
- Extended shelf life ensures reliability and convenience.
Methylmalonic acid solution (10 µg/ml) (1 x 1 ml)
- Tuning (when strongly diluted)
- Matrix-effect coverage
- Spiking samples as an internal standard
Methylmalonic acid-D3 solution (10 µg/ml) (1 x 1 ml)
- Tuning (when strongly diluted)
- Matrix-effect coverage
- Spiking samples as an internal standard
Large quantities of Calibrators or Controls (for HPLC or LC-MS)
- Maintain consistent measurement values over an extended period.
- Most of our standards are stable for multiple years, so you can safely stock up.
- Reduction of necessary and time-consuming batch verifications.
- Identical products available for multiple machines and laboratories within your organization.
- Possibilities to alter the panel composition.
- Options for personalization (levels, values, sample volumes, labeling).
- Customized inventory management.
- IVD or RUO depending on your requirements and location
Custom made Calibrators & Controls
- Clinical Diagnostics
- Clinical Research
- Forensics
- Pharmaceutical Analysis
- Environmental Analysis
- Food Safety / Food Quality
- Any other laboratory that requires independent customized high quality standards