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Methylmalonic acid (MMA) LC-MS
Methylmalonic Acid (MMA) LC-MS Reagens Kit
Advantages of the Diagnotix Methylmalonic (MMA) LC-MS method:
- Reversed Phase method with excellent separation between MMA and Succinyl Acid
- No need for derivatisation and positive mode measurements
- MMA-D3 internal standard lyophilized
- Six-points calibrator set in human serum
- Complex Precision CV is < 5%
- Relative long column life time
Component:
- Methylmalonic Acid (Methylmalonate, MMA)
At a glance:
- Matrix: serum/plasma
- Technique: LC-MS/MS
- Internal Standard: Methylmalonic Acid-D3
Intended use
The Diagnotix MMA LC-MS kit is developed to determine the patient's MMA concentration as a basis for further diagnostics.
Technical benefits
- Based on Reversed-Phase LC-MS/MS technology. LC-MS/MS allows the possibility to measure MMA from blood.
- Stable retention time while having complete separation of MMA and its isobar, succinic acid.
- The simple setup of the method allows for easy integration of other methodologies before or after, on the same LC-MS/MS system, without the need for extended conditioning time.
- Due to the chromatographic stability you achieve high data quality and win time in data-analysis.
- Refer to the technical details for the outstanding validation scores.
Why choosing for blood sampling over urine sampling
Serum samples are generally used for MMA determination, as this matrix is used for parallel cobalamin level tests. The advantage of determination from serum therefore is the sample availability. Furthermore nutrition seems to have less influence on the MMA serum level than in case with urine. For determination in urine additional measurement of creatinine is also necessary.
Especially interesting for: Laboratories running large numbers of samples once or more times per week
Contact Us for More Information
Interested in learning more about our MMA analysis? Contact us via the contact form below. We looking forward to hear from you!
Included in kit (up to 300 assays):
- 1001 CAL M MMA, Calibrator Set (Calibrator 1 – 6), 6 x 2 x 500 µl
- 1008 M MMA, Internal Standard, 3 x 6 ml
- 1009 M MMA, Deproteinization Solution, 3 x 55 ml
- 1010a M MMA, Mobile Phase I, 1 x 500 ml
- 1011 M MMA, Mobile Phase II, 1 x 300 ml
- 1012 M MMA, Solvent, 3 x 30 ml
- 10001 M MMA, Autosampler Washing Solution, 1 x 1000 ml
See also
- Methylmalonic Acid calibrator set (6 x 2 x 500 µl)
- Methylmalonic Acid Control set (3 x 3 x 500 µl)
- Methylmalonic Acid Internal Standard (10 x 6 ml)
- Methylmalonic acid solution (10 µg/ml) (1 x 1 ml)
- Methylmalonic acid-D3 solution (10 µg/ml) (1 x 1 ml)
- Large quantities of Calibrators or Controls (for HPLC or LC-MS)
- Custom made Calibrators & Controls
Chromatograms
Technical data
| Applicable for at Least: | Waters TQ-Sµ, Shimadzu LC-8050, Sciex 4500 |
|---|---|
| Linearity (nmol/l) | 5000 |
| Accuracy | Passes (<15% CV) |
| Simple Precision (CV %) | 2.0 |
| Complex Precision (CV %) | 4.9 |
| Ionization | Electrospray |
| MS/MS mode | Negative |
Important Notice:
The information provided on this website alone is not sufficient for the correct and safe operation of these medical devices. Always refer to the applicable Instructions for Use and accompanying documentation, including any warnings and safety notices. All essential documentation is available in the Download Centre on this website.
Please note that system-specific conditions may influence results. If you have any questions or require technical support, we encourage you to contact us to discuss your specific setup or requirements.
Included in this set
Product downloads
More info?
Contact us for more information about this product using the contact form or the contact information below.
Contact details
De Plassen 4
9902 SE Appingedam
The Netherlands