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LCMS - Cortisol & Cortisone in Urine Dilute and Shoot
Cortisol & Cortisone Urine LC-MS D&S reagens kit
The Cortisol & Cortisone reagents set from diagnotix • •• can determine the concentrations of both cortisol and cortisone in urine or saliva.
Advantages of the Diagnotix Cortisol & Cortisone Urine LC-MS D&S method:
- 6-point calibrator based on Human urine like matrix
- Internal standard with deuterium labeled cortisol and cortisone
- Our extremely simple sample preparation process helps you save both time and costs, making your workflow more efficient and cost-effective
- Easy to automate
Intended use
The Diagnotix Cortisol & Cortisone LC-MS D&S Kit is specifically designed to measure cortisol and cortisone concentrations in patient samples, providing a reliable foundation for further diagnostic evaluations.
Technical benefits
- Simplified sample preparation: A simple dilution is enough for fast and reliable analyses.
- Savings: Saving preparation time and eliminate the need of expensive SPE-tubes and workflow.
Especially interesting for:
- Laboratories processing relatively small sample volumes on a biweekly or monthly basis.
- Laboratories with sufficient LC-MS knowledge that understands the importance of optimized and frequent LC-MS cleanup schedules.
- Facilities operating multiple LC-MS systems, allowing for dedicated systems for specific methodologies.
Contact Us for More Information
Interested in learning more about our Cortisol&Cortisone D&S analysis? Contact us via the contact form below. We looking forward to hear from you!
Included in kit (300 assays):
- 1042 CAL M CORT, Calibrator Set (Calibrator 1 – 6), 6 x 2 x 1 ml
- 1049 M CORT, Internal Standard, 3 x 6 ml
- 1190 M CODS, Dilution Solution 3 x 100 ml
- 1191 M CODS, Mobile Phase I, 1 x 500 ml
- 1192 M CODS, Mobile Phase II, 1 x 500 ml
- 1196 M CODST, Autosampler Washing Solution, 1 x 1000 ml
Clinical relevance
The ratio between cortisol and cortisone is of clinical importance with the diagnosis of Cushing syndrome. This even indicates apparent mineralocorticoid excess, a disease where the 11β-hydroxysteroid dehydrogenase-activity is weakened significantly or is even absent.
See also
- Cortisol & Cortisone urine LC-MS Kit including SPE sample preparation for optimized signal-to-noise ratio
- Cortisol & Cortisone in Urine calibrator set
- Cortisol & Cortisone in Urine control set
- Cortisol & Cortisone Internal Standard D4 D8 (box of 10 x 6 ml)
- Cortisol solution (10 µg/ml) (1 x 1 ml)
- Cortisol-D4 solution (10 µg/ml) (1 x 1 ml)
- Cortisone solution (10 µg/ml) (1 x 1 ml)
- Cortisone-D8 solution (10 µg/ml) (1 x 1 ml)
- Large quantities of Calibrators or Controls (for HPLC or LC-MS)
- Custom made Calibrators & Controls
Chromatograms
Technical data
| Number of samples kit | 300 |
|---|---|
| Matrix | Urine |
| Sample Preparation | • Centrifuge your sample |
| • Pipette 100 µl into vial | |
| • Add 900 µl Dilution Solution and mix well | |
| • Transfer into LC-MS vial or 96-wellplate | |
| • Ready to analyze | |
| Limit of Detection (LOB) | • Cortisol: 0 nmol/l |
| • Cortisone: 0.218 nmol/l | |
| Linearity | • Cortisol: 1200 nmol/l |
| • Cortison: 3100 nmol/l | |
| Repeatability (CV%) | • Cortisol control low: 2.0 |
| • Cortisol control high: 1.0 | |
| • Cortisol patient: 1.4 | |
| • Cortisone control low: 3.0 | |
| • Cortisone control high: 2.5 | |
| • Cortison patient: 0.9 | |
| Reproducibility (CV%) | • Cortisol control low: 3.0 |
| • Cortisol control high: 2.5 | |
| • Cortisol patient: 3.2 | |
| • Cortisone control low: 2.9 | |
| • Cortisone control high: 2.6 | |
| • Cortisone patient: 3.4 | |
| Injection volume | 5 - 20 µl |
| Ionisation | ESI Postive |
| Examples of LC-MS systems | • Waters TQS-Micro or more sensitive |
| • Shimadzu 8050 of more sensitive | |
| • Sciex 5500 of more senstive |
Please Note:
Information as presented freely on this website is not sufficient for proper operation of these medical devices. Please refer to the applicable instructions for use and all accompanying information such as warning notices. All necessary documentation can be found in the download centre section of this website.For other LC-MS/MS systems than listed above, please contact us to discuss the possibilities.
More info?
Contact us for more information about this product using the contact form or the contact information below.
Contact details
De Plassen 4
9902 SE Appingedam
The Netherlands
Related products
Cortisol & Cortisone Urine LC-MS reagens kit (by SPE clean up)
- 6-point calibrator based on Human urine like matrix
- Internal standard with deuterium labeled cortisol and cortisone
- Proper product clean-up using SPE-columns
- Extremely sensitive, making it possible even the very low concentrations in saliva can be measured
- The kit does not include SPE material, allowing you to purchase it directly from the manufacturer in your preferred format at the best price to meet your laboratory's specific requirements.
Cortisol & Cortisone Conditioning Solution
-
A single vial Conditioning Solution from the Cortisol & Cortisone reagents set.
Cortisol & Cortisone Mobile Phase I
-
A single vial Mobile Phase I from the Cortisol & Cortisone reagents set.
Cortisol & Cortisone Mobile Phase II
-
A single vial Mobile Phase II from the Cortisol & Cortisone reagents set.
Cortisol & Cortisone Autosampler Washing Solution
-
A single vial Autosampler Washing Solution from the Cortisol & Cortisone reagents set.
Cortisol & Cortisone in Urine calibrator set
- 6 levels
- Urine like based material
Cortisol & Cortisone Internal Standard D4 D8 (box of 10 x 6 ml)
- Average number of samples per bottle: 100 to 120
Cortisol & Cortisone in Urine control set
- 3 levels - Low, mid, High
- Urine like based material
Cortisol solution (10 µg/ml) (1 x 1 ml)
- Tuning (when strongly diluted)
- Matrix-effect coverage
- Spiking samples as an internal standard
Cortisol-D4 solution (10 µg/ml) (1 x 1 ml)
- Tuning (when strongly diluted)
- Matrix-effect coverage
- Spiking samples as an internal standard
Cortisone-D8 solution (10 µg/ml) (1 x 1 ml)
- Tuning (when strongly diluted)
- Matrix-effect coverage
- Spiking samples as an internal standard